| Gene | Bop1 |
| Chromosome | 15 |
| Deletion Start | 76,477,218 |
| Deletion End | 76,466,777 |
| Deletion Size | 10,442 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 15
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 76,477,031 | 76,477,050 | GCAGCGTGTAGTGTCGTGTG |
| TUP | 76,477,007 | 76,477,028 | TCGCCTAGAGCCTGATCGTCCA |
| TUR | 76,476,957 | 76,476,976 | CCTCGGCGCAAGTAATGTTC |
| TDF | 76,467,506 | 76,467,529 | TGTTCTGAATGTGGTTCTTAGTGG |
| TDP | 76,467,478 | 76,467,504 | TCTAGGTGTTCCTTACGACAGAGGCCA |
| TDR | 76,467,436 | 76,467,456 | GGTAAGTGGGCAGTGCTATGG |
PCR Primers
| Primer | Sequence |
| SU | TGAGCTAGTGTCGTCGCTG |
| SD | GCTTCAGTCATCTGTCTCAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 94 bp | + | + | - |
| TDF | TDR | 94 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14289A-A8 | VGB6 | C57BL/6NTac | |
| 14289A-B4 | VGB6 | C57BL/6NTac | |
| 14289A-E2 | VGB6 | C57BL/6NTac | |
| 14289A-E3 | VGB6 | C57BL/6NTac | |
| 14289A-F7 | VGB6 | C57BL/6NTac | |
| 14289A-H7 | VGB6 | C57BL/6NTac | |
