| Gene | Abcc8 |
| Chromosome | 7 |
| Deletion Start | 46,179,946 |
| Deletion End | 46,150,733 |
| Deletion Size | 29,214 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 46,179,388 | 46,179,405 | TTCCGGTGGCCCAACTTC |
| TUP | 46,179,363 | 46,179,386 | CGACTTTGGGCAGAGCATTTCCCA |
| TUR | 46,179,315 | 46,179,335 | CCGGCACCCAAACTCTATCTC |
| TDF | 46,150,824 | 46,150,845 | GGGCATCAAACTGCTCAAACTG |
| TDP | 46,150,800 | 46,150,822 | ACGCGTGGGAGAACATCTTCTGC |
| TDR | 46,150,772 | 46,150,790 | CCTTCCTGCGCGTCATCTC |
PCR Primers
| Primer | Sequence |
| SU | AGCTGAGCCTAGTTCCAGAG |
| SD | GCACAAGTGAATTCCAAACAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 91 bp | + | + | - |
| TDF | TDR | 74 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14299A-A6 | VGB6 | C57BL/6NTac | |
| 14299A-H3 | VGB6 | C57BL/6NTac | |
| 14299A-F12 | VGB6 | C57BL/6NTac | |
