14300 Abcb4 Status: ES cell colonies screened / QC no positives
| Gene | Abcb4 |
| Chromosome | 5 |
| Deletion Start | 8,894,097 |
| Deletion End | 8,920,455 |
| Deletion Size | 26,359 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 5
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 8,894,105 | 8,894,124 | GGCAGCGAGAAACGGAACAG |
| TUP | 8,894,130 | 8,894,151 | CGCCTGGACGGCGACTTTGAAC |
| TUR | 8,894,170 | 8,894,188 | CAGTGACTCTGCGGTCCTG |
| TDF | 8,920,309 | 8,920,329 | GTGCTCTTCCTTGCTTTCCTC |
| TDP | 8,920,330 | 8,920,353 | TGCAGATCTTGAAGGGCCTCAACC |
| TDR | 8,920,361 | 8,920,381 | GCCACTGTCTGTCCACTCTTC |
PCR Primers
| Primer | Sequence |
| SU | AGGCATCGAGCAGGCTTATC |
| SD | GGACAAATCAAACTCACTGAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 84 bp | + | + | - |
| TDF | TDR | 73 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
