| Gene | A3galt2 |
| Chromosome | 4 |
| Deletion Start | 128,759,912 |
| Deletion End | 128,767,726 |
| Deletion Size | 7,815 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 128,760,567 | 128,760,586 | CAGTGCCCACTCCCTCCAAG |
| TUP | 128,760,587 | 128,760,613 | CCAGGTCTTGGGCCTTTGAGCTATGTG |
| TUR | 128,760,629 | 128,760,648 | GTGTCAGCCAGTTCCACCAG |
| TDF | 128,767,383 | 128,767,405 | CGTTAAGCGAAGGCGATTTCTAC |
| TDP | 128,767,406 | 128,767,426 | TACCACGCTGCGGTGTTTGGC |
| TDR | 128,767,440 | 128,767,458 | GCCGTCAGCTTGAGCAGTG |
PCR Primers
| Primer | Sequence |
| SU | AGGCCAAGATAGTTCCCAGG |
| SD | ATTGTCAAGGTGTGGGAACC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 82 bp | + | + | - |
| TDF | TDR | 76 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14304A-A1 | VGB6 | C57BL/6NTac | |
| 14304A-D1 | VGB6 | C57BL/6NTac | |
| 14304A-D3 | VGB6 | C57BL/6NTac | |
| 14304A-F12 | VGB6 | C57BL/6NTac | |
