Information regarding availability, timelines and delivery of materials can be found at www.komp.org or www.knockoutmouse.org.

14308 Trpm4 Status: ES cell colonies screened / QC positives

Gene	Trpm4
Chromosome	7
Deletion Start	45,333,707
Deletion End	45,303,664
Deletion Size	30,044
Genome Build	38

MGI ID	MGI:1915917
NCBI Gene ID	0
BAC ID	292L19
BAC Library	bMQ
Cassette	ZEN-Ub1
Clones Available	Yes

Sequence Downloads and Strategy Information

Primer Genomic Coordinates on Chromosome 7

Please be aware that these oligonucleotides have been validated only by Taqman assay.

Primer	Start	End	Sequence
TUF	45,332,896	45,332,913	TAGGGCTGCGCTTGTCAC
TUP	45,332,871	45,332,895	CTGGGCACAGAACAGCTCCTCCCTT
TUR	45,332,810	45,332,831	GATGGGCAGAGTTCCAGAAATG
TDF	45,304,057	45,304,077	GCCAAAGAAACGCACCAGAAG
TDP	45,304,028	45,304,051	AACAACGCTGTTCGCTGGACCACA
TDR	45,303,985	45,304,008	GGAGTCCTGAACTGTTTGCTGAAG

PCR Primers

Primer	Sequence
SU	CTCTGCTTCCTTCGTTCCTC
SD	CAACATCACACACCTGCTCC
LacInR	TTGACTGTAGCGGCTGATGTTG
LacInf	GGTAAACTGGCTCGGATTAGGG
NeoInR	TACTTTCTCGGCAGGAGCAAGGTG
NeoInF	TTCGGCTATGACTGGGCACAACAG
LacInZRev	GTCTGTCCTAGCTTCCTCACTG
NeoFwd	TCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward Primer	Reverse Primer	Length	WT	HET	KO
SU	LacZRev	bp	-	+	+
TUF	TUR	104 bp	+	+	-
TDF	TDR	93 bp	+	+	-
NeoFwd	SD	bp	-	+	+
LacInf	LacInR	210 bp	-	+	+
NeoInf	NeoInR	282 bp	-	+	+

Available Clones

ES Cell ordering information can be found at the KOMP Repository.

Clone Name	Parental ES Cell	Mouse Strain	Cassette Copy Number
14308A-B10	VGB6	C57BL/6NTac
14308A-C2	VGB6	C57BL/6NTac
14308A-G9	VGB6	C57BL/6NTac
14308A-H7	VGB6	C57BL/6NTac