| Gene | Cntn1 |
| Chromosome | 15 |
| Deletion Start | 92,216,390 |
| Deletion End | 92,254,130 |
| Deletion Size | 37,741 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 15
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 92,216,716 | 92,216,738 | GACAGCATGGCTTAAATCAACAC |
| TUP | 92,216,746 | 92,216,775 | TGTAGAATCGGGAGAGGTGGTTGGTATTTC |
| TUR | 92,216,779 | 92,216,802 | GGTATCTTCGATTCTAGGCAGGTG |
| TDF | 92,253,157 | 92,253,178 | GCAGTGGACTAGATGGGAGAAC |
| TDP | 92,253,180 | 92,253,207 | TGGAAGGTAAAGGGTATGCCATCACTGG |
| TDR | 92,253,209 | 92,253,231 | CACAGAGTCTCATAGCTGGTCAC |
PCR Primers
| Primer | Sequence |
| SU | AAACCTGGTGATTTGCCATC |
| SD | TCTAGACTGTTTTCAACAATTCAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 87 bp | + | + | - |
| TDF | TDR | 75 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14309B-G11 | VGB6 | C57BL/6NTac | |
