| Gene | Zmynd17 |
| Chromosome | 14 |
| Deletion Start | 20,487,658 |
| Deletion End | 20,483,060 |
| Deletion Size | 4,599 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 14
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 20,487,583 | 20,487,605 | GATTGAAATCCCACCCACAGAAG |
| TUP | 20,487,557 | 20,487,582 | TGTCTCCAGTATCTCCAGCCCTCTCA |
| TUR | 20,487,512 | 20,487,534 | GTCCAAGGAAATGAAGCCAAGTG |
| TDF | 20,483,990 | 20,484,009 | CTCCAACAGGCCACACTTTC |
| TDP | 20,483,945 | 20,483,970 | AGGCCAAGCACCCATACACATGATTC |
| TDR | 20,483,874 | 20,483,893 | CGCCCTAAGACCCAGCTTTG |
PCR Primers
| Primer | Sequence |
| SU | TGAGCTACCATGTGCCCAG |
| SD | TCAGAAATGAGTCTGGTAATG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 94 bp | + | + | - |
| TDF | TDR | 136 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14311E-C1 | VGB6 | C57BL/6NTac | |
| 14311E-G5 | VGB6 | C57BL/6NTac | |
| 14311E-G12 | VGB6 | C57BL/6NTac | |
