| Gene | Tnrc6c |
| Chromosome | 11 |
| Deletion Start | 117,700,765 |
| Deletion End | 117,740,004 |
| Deletion Size | 39,240 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 117,701,086 | 117,701,104 | GCCTCCTGAGGATGGACTG |
| TUP | 117,701,105 | 117,701,128 | TGCTCAGCCCAGAGTAGCCCTATC |
| TUR | 117,701,132 | 117,701,151 | TCCTGGTTCTCCCTGGTATC |
| TDF | 117,739,807 | 117,739,824 | TTGCCACCTCCTGTCCTG |
| TDP | 117,739,825 | 117,739,852 | TGCTCGTTCGTTCTCAGTGTTGATCTCC |
| TDR | 117,739,887 | 117,739,906 | CCACGTTTGTCCATGTCCAC |
PCR Primers
| Primer | Sequence |
| SU | AGCAGCACCCTCCTACATAC |
| SD | TGTCAGGTTCACAGGCTAAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 66 bp | + | + | - |
| TDF | TDR | 100 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14315C-C9 | VGB6 | C57BL/6NTac | |
| 14315C-H2 | VGB6 | C57BL/6NTac | |
| 14315C-G9 | VGB6 | C57BL/6NTac | |
