| Gene | Cd151 |
| Chromosome | 7 |
| Deletion Start | 141,469,265 |
| Deletion End | 141,470,793 |
| Deletion Size | 1,529 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 141,469,474 | 141,469,492 | CCTGGCTGTCATGGCAGTG |
| TUP | 141,469,497 | 141,469,521 | TCTGGACACTGGCCCTCAAGAGTGA |
| TUR | 141,469,522 | 141,469,543 | TGAGGCCAGCAGACTAATGTAG |
| TDF | 141,470,087 | 141,470,107 | TTTGCCTTTGCCCAGAGTTTG |
| TDP | 141,470,109 | 141,470,133 | TCCTACCCACAGCTGAACACAGAGC |
| TDR | 141,470,167 | 141,470,185 | TGGCCTGACTGGTGGTATC |
PCR Primers
| Primer | Sequence |
| SU | CAGCAGGAGTGAGTCAGCAG |
| SD | GTACGCTGCTTCGAGCTTTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 70 bp | + | + | - |
| TDF | TDR | 99 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14318A-D1 | VGB6 | C57BL/6NTac | |
| 14318A-G6 | VGB6 | C57BL/6NTac | |
| 14318A-E4 | VGB6 | C57BL/6NTac | |
| 14318A-H12 | VGB6 | C57BL/6NTac | |
| 14318A-A10 | VGB6 | C57BL/6NTac | |
