| Gene | Notch2 |
| Chromosome | 3 |
| Deletion Start | 98,013,702 |
| Deletion End | 98,047,805 |
| Deletion Size | 34,104 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 3
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 98,014,136 | 98,014,153 | TGCTGCGGTTCCAGTGTG |
| TUP | 98,014,162 | 98,014,186 | CCCAGGAATGTGGTGTGACGATCCC |
| TUR | 98,014,187 | 98,014,205 | TGCCAGGTTGGAGGAGTTG |
| TDF | 98,047,267 | 98,047,288 | TGGGTCCTGTTAGTGGATGTTG |
| TDP | 98,047,294 | 98,047,318 | TTTGGCTGCTTACTAAAGTGGCCCA |
| TDR | 98,047,331 | 98,047,351 | AGCTGCTTGGCTTCAGGTTAC |
PCR Primers
| Primer | Sequence |
| SU | CTGCTCACTCAGATCCCTCC |
| SD | ATAGATATTACTAGCCACTCAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 70 bp | + | + | - |
| TDF | TDR | 85 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14342A-A6 | VGB6 | C57BL/6NTac | |
| 14342A-G7 | VGB6 | C57BL/6NTac | |
