| Gene | Slc39a13 |
| Chromosome | 2 |
| Deletion Start | 91,068,728 |
| Deletion End | 91,062,849 |
| Deletion Size | 5,880 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 91,068,678 | 91,068,697 | CAGGCCAAAGGCTCCTCTTC |
| TUP | 91,068,654 | 91,068,676 | TCACTGTCCTTGCCCTGGAGCTC |
| TUR | 91,068,634 | 91,068,653 | GAACCTCCAGCCCTTTCCAG |
| TDF | 91,063,119 | 91,063,136 | TCACCTCGGGAGGCTTTC |
| TDP | 91,063,088 | 91,063,113 | ATCGCCCTGGTGAATGTGTTACCTGA |
| TDR | 91,063,061 | 91,063,080 | CACCACGGGTCGTCTTCTTC |
PCR Primers
| Primer | Sequence |
| SU | ACCACGCCATCAAGCTAAAC |
| SD | CAAGGAGGAACAACTTTGGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 64 bp | + | + | - |
| TDF | TDR | 76 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14345A-B7 | VGB6 | C57BL/6NTac | |
| 14345A-B10 | VGB6 | C57BL/6NTac | |
| 14345A-A4 | VGB6 | C57BL/6NTac | |
| 14345A-B11 | VGB6 | C57BL/6NTac | |
| 14345A-E10 | VGB6 | C57BL/6NTac | |
| 14345A-F9 | VGB6 | C57BL/6NTac | |
