| Gene | Mos |
| Chromosome | 4 |
| Deletion Start | 3,871,811 |
| Deletion End | 3,870,752 |
| Deletion Size | 1,060 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 3,871,531 | 3,871,554 | GGCCATCAAGCAAGTAAACAAGTG |
| TUP | 3,871,507 | 3,871,530 | CACCAAGGACCTACGTGCATCCCA |
| TUR | 3,871,456 | 3,871,474 | ATGTTGTCGTGGCGTAGTC |
| TDF | 3,871,035 | 3,871,054 | CGCCCAAAGCTGACATCTAC |
| TDP | 3,871,005 | 3,871,031 | TTTGGAATCACCCTGTGGCAGATGACC |
| TDR | 3,870,972 | 3,870,991 | CTGAGGTTCGCCGGAGTAAG |
PCR Primers
| Primer | Sequence |
| SU | AAGTGTGCACCAAGTTCACTG |
| SD | CAGGAACAAACCTTACTACAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 99 bp | + | + | - |
| TDF | TDR | 83 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14348A-A7 | VGB6 | C57BL/6NTac | |
| 14348A-E2 | VGB6 | C57BL/6NTac | |
| 14348A-E7 | VGB6 | C57BL/6NTac | |
| 14348A-F10 | VGB6 | C57BL/6NTac | |
