| Gene | Nipal4 |
| Chromosome | 11 |
| Deletion Start | 46,166,273 |
| Deletion End | 46,150,059 |
| Deletion Size | 16,215 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 46,165,387 | 46,165,411 | GAGGACAAGTTGGAGAAGTGAGTTC |
| TUP | 46,165,348 | 46,165,370 | TTCCAGGGCCAGAACTTGGGAGC |
| TUR | 46,165,323 | 46,165,343 | GATGGCTCCTTGGGTAAAGTC |
| TDF | 46,150,586 | 46,150,605 | CTTCCAGGGCTTACCAGTTG |
| TDP | 46,150,565 | 46,150,585 | TGCGGCACCCTCTCCCTTACA |
| TDR | 46,150,544 | 46,150,562 | GACCCAGGATGAGGGACAG |
PCR Primers
| Primer | Sequence |
| SU | GCCATCCGGAGTGTATCAAC |
| SD | CTACACACCTTGGCATGACG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 89 bp | + | + | - |
| TDF | TDR | 62 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14349A-C11 | VGB6 | C57BL/6NTac | |
| 14349A-D1 | VGB6 | C57BL/6NTac | |
| 14349A-F9 | VGB6 | C57BL/6NTac | |
| 14349A-H3 | VGB6 | C57BL/6NTac | |
