| Gene | Arhgef10 |
| Chromosome | 8 |
| Deletion Start | 14,925,187 |
| Deletion End | 14,954,924 |
| Deletion Size | 29,738 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 8
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 14,925,611 | 14,925,629 | GACTGGGCCAGCATTCTTC |
| TUP | 14,925,631 | 14,925,656 | CTGATTCCTTCCCTGTGGGCAGTTCA |
| TUR | 14,925,690 | 14,925,710 | CCGTCCTCTGTGTCTATCAAC |
| TDF | 14,954,421 | 14,954,442 | CCAATTTCCGACAGGTTGTGAG |
| TDP | 14,954,448 | 14,954,474 | ACATTCTGGGCTCCATTGTTGAGAGCG |
| TDR | 14,954,479 | 14,954,500 | CCGGAGAGCATCTACATAGTTC |
PCR Primers
| Primer | Sequence |
| SU | TGTTGGTTGAGATGGCTCTG |
| SD | ACGTACACTTAACCCTTTCTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 100 bp | + | + | - |
| TDF | TDR | 80 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14354A-G10 | VGB6 | C57BL/6NTac | |
| 14354C-C6 | VGB6 | C57BL/6NTac | |
| 14354C-G8 | VGB6 | C57BL/6NTac | |
