| Gene | Tnni3 |
| Chromosome | 7 |
| Deletion Start | 4,522,312 |
| Deletion End | 4,518,360 |
| Deletion Size | 3,953 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 4,521,597 | 4,521,616 | GGCGTGAGCGTAGTGGTTAG |
| TUP | 4,521,561 | 4,521,589 | TAGATAGGCAGTGCGTAGCTCTAGTCGGA |
| TUR | 4,521,534 | 4,521,554 | CCTCGGTCCCATCTACCAATC |
| TDF | 4,518,518 | 4,518,538 | ACAGGCTTCCAGCACAGAATG |
| TDP | 4,518,485 | 4,518,513 | AGCAAGCTAGAGGGTAGATAGAGACCAGA |
| TDR | 4,518,450 | 4,518,471 | TCCTGCAAGTGGAAAGTCAGAG |
PCR Primers
| Primer | Sequence |
| SU | TGAGGGACCTAGGTGTGAGG |
| SD | GCTTGGCACGTTCTATGCTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 83 bp | + | + | - |
| TDF | TDR | 89 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14359B-A8 | VGB6 | C57BL/6NTac | |
| 14359B-D5 | VGB6 | C57BL/6NTac | |
| 14359B-F9 | VGB6 | C57BL/6NTac | |
| 14359B-H4 | VGB6 | C57BL/6NTac | |
