| Gene | Spata7 |
| Chromosome | 12 |
| Deletion Start | 98,649,510 |
| Deletion End | 98,669,668 |
| Deletion Size | 20,159 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 12
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 98,628,508 | 98,628,530 | GCCTGTATCACTTGGGTATCTTC |
| TUP | 98,628,532 | 98,628,557 | AGGCGGAATACTAGCCTGGTCTTTGA |
| TUR | 98,628,594 | 98,628,611 | CTGAGCGCCGTTTCTGTG |
| TDF | 98,647,943 | 98,647,961 | TGTTGGCATGGCTTTGCTG |
| TDP | 98,647,964 | 98,647,992 | TTGCTTGCTCATTTGAGCTGAGTTATTGC |
| TDR | 98,648,000 | 98,648,019 | GGCCAGGAGTTCAGGGACAG |
PCR Primers
| Primer | Sequence |
| SU | GCTGGCAGAAGCAGATATGAC |
| SD | CTGTCTCTGGCTAGCGAATG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 104 bp | + | + | - |
| TDF | TDR | 77 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14363A-E1 | VGB6 | C57BL/6NTac | |
| 14363A-E3 | VGB6 | C57BL/6NTac | |
| 14363A-F1 | VGB6 | C57BL/6NTac | |
