| Gene | Klkb1 |
| Chromosome | 8 |
| Deletion Start | 45,294,128 |
| Deletion End | 45,269,726 |
| Deletion Size | 24,403 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 8
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 45,293,633 | 45,293,653 | ACCTGCTTTGGGTTTCACAAG |
| TUP | 45,293,604 | 45,293,629 | AGTATCCCTTTGGCAGTCTGGAGGGA |
| TUR | 45,293,573 | 45,293,596 | AAAGAGCACTGACATCGAGTGTTG |
| TDF | 45,269,841 | 45,269,859 | ACCTGCCCTCAAGGCAAAC |
| TDP | 45,269,814 | 45,269,839 | AGAGCTCCAGGATTCTCGGCTGTAAA |
| TDR | 45,269,775 | 45,269,799 | CCAATGATACGGATGTGAGGTAGAC |
PCR Primers
| Primer | Sequence |
| SU | ATTTGGATCTTGGGAAGCATG |
| SD | ACATGAGAATGGTCCCAAGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 81 bp | + | + | - |
| TDF | TDR | 85 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14365A-H7 | VGB6 | C57BL/6NTac | |
| 14365A-E7 | VGB6 | C57BL/6NTac | |
| 14365A-G7 | VGB6 | C57BL/6NTac | |
| 14365A-E2 | VGB6 | C57BL/6NTac | |
