| Gene | Aqp2 |
| Chromosome | 15 |
| Deletion Start | 99,579,156 |
| Deletion End | 99,584,136 |
| Deletion Size | 4,981 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 15
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 99,579,997 | 99,580,019 | TCTCTGCCTCAACCAACTATCTG |
| TUP | 99,580,029 | 99,580,054 | ATGGGCTTTGTCTCACCAACCAAGAA |
| TUR | 99,580,056 | 99,580,076 | CCGTCTCCCTTTCCAAGTCTG |
| TDF | 99,583,685 | 99,583,709 | CGTGATGACCTTTATTGCATAAGTC |
| TDP | 99,583,715 | 99,583,738 | AATACCAGAGGCACAGGAGGGCCA |
| TDR | 99,583,746 | 99,583,766 | ACGCTGCTATCCCGGTTAGTG |
PCR Primers
| Primer | Sequence |
| SU | CTCCTCTGCAGGAACTGGTG |
| SD | GGCCAAAGCAAGAATGAAAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 80 bp | + | + | - |
| TDF | TDR | 82 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14367A-D12 | VGB6 | C57BL/6NTac | |
| 14367A-E3 | VGB6 | C57BL/6NTac | |
| 14367A-G8 | VGB6 | C57BL/6NTac | |
| 14367A-F5 | VGB6 | C57BL/6NTac | |
| 14367A-E5 | VGB6 | C57BL/6NTac | |
| 14367A-E12 | VGB6 | C57BL/6NTac | |
