| Gene | 1810013D10Rik |
| Chromosome | 5 |
| Deletion Start | 53,267,247 |
| Deletion End | 53,277,934 |
| Deletion Size | 10,688 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 5
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 53,267,273 | 53,267,294 | GCTCATATTCGGAGGCTTCATC |
| TUP | 53,267,306 | 53,267,332 | CGCCGCCTTCTATCCCATCTACTTCCG |
| TUR | 53,267,352 | 53,267,373 | TCAGAGGTCACTCACGGTATTC |
| TDF | 53,277,163 | 53,277,184 | CGAGCTGGTATTGTCCAGGAAG |
| TDP | 53,277,186 | 53,277,210 | TGTGCAACCGCCAGGTAAACTGAAA |
| TDR | 53,277,249 | 53,277,269 | ACCACCCACTCCACAGCAAAG |
PCR Primers
| Primer | Sequence |
| SU | GAGAAGGAAGGACCCAGACC |
| SD | TGAAACATCCTCCAAGAGCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 101 bp | + | + | - |
| TDF | TDR | 107 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14369A-A10 | VGB6 | C57BL/6NTac | |
| 14369A-B5 | VGB6 | C57BL/6NTac | |
| 14369A-G11 | VGB6 | C57BL/6NTac | |
| 14369A-B12 | VGB6 | C57BL/6NTac | |
| 14369A-G3 | VGB6 | C57BL/6NTac | |
