| Gene | Uqcr10 |
| Chromosome | 11 |
| Deletion Start | 4,704,303 |
| Deletion End | 4,702,097 |
| Deletion Size | 2,207 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 4,703,379 | 4,703,400 | CCGGAGCTAGATGGTAGACAAG |
| TUP | 4,703,353 | 4,703,376 | TTGGCCTTAGACTCACCCTCCTGC |
| TUR | 4,703,322 | 4,703,343 | GGCCTACAATCCCAACACTCAG |
| TDF | 4,702,285 | 4,702,307 | TCTCTGCTCTTCTCTTTGGACAG |
| TDP | 4,702,239 | 4,702,267 | TGTGAGGACAAAGGTTAACGGTGAACTGC |
| TDR | 4,702,178 | 4,702,200 | ACTCCTTGTTCTCATACTTGTGC |
PCR Primers
| Primer | Sequence |
| SU | CTCCTCCTCTTGAGTCCTCG |
| SD | CAAACTAGGATTCTTGGGACG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 79 bp | + | + | - |
| TDF | TDR | 130 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14374A-A9 | VGB6 | C57BL/6NTac | |
| 14374A-B9 | VGB6 | C57BL/6NTac | |
| 14374A-B12 | VGB6 | C57BL/6NTac | |
| 14374A-C12 | VGB6 | C57BL/6NTac | |
| 14374A-B11 | VGB6 | C57BL/6NTac | |
| 14374A-C3 | VGB6 | C57BL/6NTac | |
