| Gene | Pon1 |
| Chromosome | 6 |
| Deletion Start | 5,193,746 |
| Deletion End | 5,181,444 |
| Deletion Size | 12,303 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 6
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 5,192,937 | 5,192,955 | GGGCACAACACTGCATCTC |
| TUP | 5,192,907 | 5,192,935 | AACCACTGAATGCTCCAATAAACCAGCCA |
| TUR | 5,192,864 | 5,192,887 | GCATTCCAGGCTTCTTGTTAGTAG |
| TDF | 5,181,977 | 5,181,995 | TACAAGCAGCCACAGCATC |
| TDP | 5,181,955 | 5,181,975 | AGTCCAGCTCCCACACCCTCA |
| TDR | 5,181,907 | 5,181,930 | TGACAGTTCAAATGATCGTCACAG |
PCR Primers
| Primer | Sequence |
| SU | TCTGTGCTCGTAATTGGCTG |
| SD | GACTCTCTGAGATTGTTCTTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 92 bp | + | + | - |
| TDF | TDR | 89 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14386A-D10 | VGB6 | C57BL/6NTac | |
| 14386B-F2 | VGB6 | C57BL/6NTac | |
| 14386C-E1 | VGB6 | C57BL/6NTac | |
| 14386B-E3 | VGB6 | C57BL/6NTac | |
