| Gene | Trim71 |
| Chromosome | 9 |
| Deletion Start | 114,563,114 |
| Deletion End | 114,524,959 |
| Deletion Size | 38,156 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 9
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 114,562,178 | 114,562,199 | TCTCGGAGAACCAGGATTTGAG |
| TUP | 114,562,152 | 114,562,176 | ACGGTTTCCTTCTCTGCATGTCCGA |
| TUR | 114,562,128 | 114,562,150 | GGGAATCTCGTGTTTTGGGACTC |
| TDF | 114,525,455 | 114,525,475 | GGCTCACCGTACTGCTTCTTG |
| TDP | 114,525,427 | 114,525,451 | TTGCTTCACATACAGCGTGACCACA |
| TDR | 114,525,397 | 114,525,420 | CTATGGTTCTCTGCTCTGGGATTG |
PCR Primers
| Primer | Sequence |
| SU | ACTTGGACTGCTGCGACTC |
| SD | GTGCACCAAATACCCAAACG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 72 bp | + | + | - |
| TDF | TDR | 79 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14408A-B6 | VGB6 | C57BL/6NTac | |
| 14408A-C6 | VGB6 | C57BL/6NTac | |
| 14408A-F12 | VGB6 | C57BL/6NTac | |
| 14408A-G3 | VGB6 | C57BL/6NTac | |
| 14408A-G7 | VGB6 | C57BL/6NTac | |
| 14408A-H11 | VGB6 | C57BL/6NTac | |
