| Gene | Rspo1 |
| Chromosome | 4 |
| Deletion Start | 124,991,336 |
| Deletion End | 125,008,549 |
| Deletion Size | 17,214 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 124,991,929 | 124,991,946 | AGCTGAAGCCAGCACACC |
| TUP | 124,991,949 | 124,991,975 | TCTGAGTGGCAGTGACCTTACTGTGTA |
| TUR | 124,992,004 | 124,992,022 | TCAGGGCTACAGGCATTTC |
| TDF | 125,008,072 | 125,008,091 | CTGATGAGTGGCAGGGAGTG |
| TDP | 125,008,101 | 125,008,126 | ACTGTCCCATTTGTCCTGAACAGCCA |
| TDR | 125,008,140 | 125,008,161 | CAGCCCTTGTACCCTAAAGACC |
PCR Primers
| Primer | Sequence |
| SU | TCTACAAAGTCCAGGGCAGG |
| SD | AAGCGCTTTCCCTCTCTCTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 94 bp | + | + | - |
| TDF | TDR | 90 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14412A-C1 | VGB6 | C57BL/6NTac | |
| 14412A-G4 | VGB6 | C57BL/6NTac | |
| 14412A-C5 | VGB6 | C57BL/6NTac | |
| 14412A-D12 | VGB6 | C57BL/6NTac | |
| 14412A-C9 | VGB6 | C57BL/6NTac | |
