| Gene | Ndufs4 |
| Chromosome | 13 |
| Deletion Start | 114,388,014 |
| Deletion End | 114,351,453 |
| Deletion Size | 36,562 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 13
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 114,387,990 | 114,388,009 | GCGAAGGGCAATGGCTACAG |
| TUP | 114,387,964 | 114,387,988 | TGCCGTTTCCGTCTGTAGAGTTCCA |
| TUR | 114,387,909 | 114,387,932 | GACCTGGGAACCCTGAATGAATAG |
| TDF | 114,351,544 | 114,351,567 | CATGGCAAGAGCATCCTTTAACTC |
| TDP | 114,351,519 | 114,351,543 | CACGTCTTGCACTGCAGGTTGTTGA |
| TDR | 114,351,497 | 114,351,517 | GCCAGCTTCCAAGTGGATGTG |
PCR Primers
| Primer | Sequence |
| SU | GGCATCATTTGCATTCACTG |
| SD | GCTCACATTACCACACAGTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 101 bp | + | + | - |
| TDF | TDR | 71 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14414A-G3 | VGB6 | C57BL/6NTac | |
| 14414A-E7 | VGB6 | C57BL/6NTac | |
| 14414A-C1 | VGB6 | C57BL/6NTac | |
| 14414A-G5 | VGB6 | C57BL/6NTac | |
