14415   BC060267     Status: ES cell colonies screened / QC positives

GeneBC060267
Chromosome6
Deletion Start115,838,129
Deletion End115,810,961
Deletion Size27,169
Genome Build38
MGI IDMGI:2681834
NCBI Gene ID212516
BAC ID356K22
BAC LibrarybMQ
CassetteZEN-Ub1
Clones AvailableYes

Primer Genomic Coordinates on Chromosome 6

Please be aware that these oligonucleotides have been validated only by Taqman assay.

PrimerStartEndSequence
TUF115,837,571115,837,590GGAGAAGCTGCCTGGTGTAG
TUP115,837,540115,837,566TTGACACTTAGAGGAGTGACCAGGACA
TUR115,837,504115,837,524CCTTCCTTTCTGGCCGTCATC
TDF115,811,185115,811,205TCAGACACCCATCCCAATCAC
TDP115,811,163115,811,184TTCTGGCCCGGTCACCTTCTGG
TDR115,811,131115,811,151GGCCACAGTAGGCAGGTAGAG

PCR Primers

PrimerSequence
SUTTTCCACCAAACGGCTAAAC
SDTATCCCAGGATGAGAGGTGG
LacInRTTGACTGTAGCGGCTGATGTTG
LacInfGGTAAACTGGCTCGGATTAGGG
NeoInRTACTTTCTCGGCAGGAGCAAGGTG
NeoInFTTCGGCTATGACTGGGCACAACAG
LacInZRevGTCTGTCCTAGCTTCCTCACTG
NeoFwdTCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward PrimerReverse PrimerLengthWTHETKO
SULacZRev bp-++
TUFTUR87 bp++-
TDFTDR75 bp++-
NeoFwdSD bp-++
LacInfLacInR210 bp-++
NeoInfNeoInR282 bp-++

Available Clones

ES Cell ordering information can be found at the KOMP Repository.
Clone NameParental ES CellMouse StrainCassette Copy Number
14415A-F5VGB6C57BL/6NTac
14415A-G12VGB6C57BL/6NTac
14415A-H3VGB6C57BL/6NTac
VELOCIGENE KOMP DESIGN DIAGRAMS
VelociGene KOMP Design
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VelociGene Genotyping Strategy

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