| Gene | Phex |
| Chromosome | X |
| Deletion Start | 157,414,753 |
| Deletion End | 157,403,372 |
| Deletion Size | 11,382 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome X
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 157,414,699 | 157,414,717 | GGGACCAACCGAGGCATTC |
| TUP | 157,414,670 | 157,414,695 | TTGCACTGGCCCTGTTTATTGGTGGC |
| TUR | 157,414,642 | 157,414,661 | GAAAGAGCAGTGTGCCCAAC |
| TDF | 157,403,870 | 157,403,890 | TGGGAAGCAGAGCCAAGTTAG |
| TDP | 157,403,832 | 157,403,859 | AACATGTGGGATGGAAAGAAGGCCAACA |
| TDR | 157,403,776 | 157,403,797 | TTGGCCCTACATCTTTGGAGTG |
PCR Primers
| Primer | Sequence |
| SU | TTGGATGCAGACTGTCCTTG |
| SD | TATCTTTGTGCTCCCAAACTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 76 bp | + | + | - |
| TDF | TDR | 115 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14421A-E4 | VGB6 | C57BL/6NTac | |
| 14421A-E6 | VGB6 | C57BL/6NTac | |
