| Gene | Jun |
| Chromosome | 4 |
| Deletion Start | 95,051,268 |
| Deletion End | 95,050,211 |
| Deletion Size | 1,058 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 95,051,204 | 95,051,223 | TCGTTCCTCCAGTCCGAGAG |
| TUP | 95,051,180 | 95,051,203 | CGGTGCCTACGGCTACAGTAACCC |
| TUR | 95,051,086 | 95,051,107 | CGTGAGAAGGTCCGAGTTCTTG |
| TDF | 95,050,373 | 95,050,391 | ACTCCGAGCTGGCATCCAC |
| TDP | 95,050,346 | 95,050,370 | CCAACATGCTCAGGGAACAGGTGGC |
| TDR | 95,050,283 | 95,050,302 | TGCTGCGTTAGCATGAGTTG |
PCR Primers
| Primer | Sequence |
| SU | GAGAATTCCGTGACGACTGG |
| SD | TAGCCTGGCACTTACAAGCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 138 bp | + | + | - |
| TDF | TDR | 109 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14429A-A9 | VGB6 | C57BL/6NTac | |
| 14429A-F5 | VGB6 | C57BL/6NTac | |
