| Gene | Rdh12 |
| Chromosome | 12 |
| Deletion Start | 79,209,044 |
| Deletion End | 79,222,154 |
| Deletion Size | 13,111 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 12
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 79,209,589 | 79,209,610 | GAAAGGTTAGGAGCCAGGTGTG |
| TUP | 79,209,613 | 79,209,640 | TGTACGTGCCTTTAATCCGAGCACTCAG |
| TUR | 79,209,672 | 79,209,693 | CGCTCTGTAGATCAGTCTGTCC |
| TDF | 79,221,684 | 79,221,705 | GCTTTGCTTTGTCAAGATGGTC |
| TDP | 79,221,709 | 79,221,736 | TTGCAGTCCAGGATAGGTTCCAAGTCAC |
| TDR | 79,221,761 | 79,221,781 | CAGAGGCAGGAAGATCAGGAG |
PCR Primers
| Primer | Sequence |
| SU | AGCGTGTTGAGGAGGAACTC |
| SD | CATACAATGGCTCCAGGATG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 105 bp | + | + | - |
| TDF | TDR | 98 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14431A-A7 | VGB6 | C57BL/6NTac | |
| 14431A-C10 | VGB6 | C57BL/6NTac | |
