| Gene | Chn1 |
| Chromosome | 2 |
| Deletion Start | 73,659,928 |
| Deletion End | 73,624,119 |
| Deletion Size | 35,810 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 73,659,292 | 73,659,312 | CCGATTCTGTCCGAGGCTTTG |
| TUP | 73,659,266 | 73,659,290 | TGTAGCAGGCATGTTCAGTCGCATC |
| TUR | 73,659,236 | 73,659,256 | ACGGGAAATGCACACCATCTG |
| TDF | 73,624,929 | 73,624,948 | CGCTCGTGAAAGCTCACATC |
| TDP | 73,624,904 | 73,624,928 | ACCAAGCGGCCAATGGTGGTAGACA |
| TDR | 73,624,852 | 73,624,869 | GGCAGCCTTCGTCCAAAC |
PCR Primers
| Primer | Sequence |
| SU | ATGCTGCATGCTTTGTATGG |
| SD | TCTTTCTCACCAGGAAATCTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 77 bp | + | + | - |
| TDF | TDR | 97 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14437A-D10 | VGB6 | C57BL/6NTac | |
| 14437A-E5 | VGB6 | C57BL/6NTac | |
| 14437A-E9 | VGB6 | C57BL/6NTac | |
| 14437A-E8 | VGB6 | C57BL/6NTac | |
| 14437A-F3 | VGB6 | C57BL/6NTac | |
| 14437A-C3 | VGB6 | C57BL/6NTac | |
