| Gene | Trim31 |
| Chromosome | 17 |
| Deletion Start | 36,898,357 |
| Deletion End | 36,909,762 |
| Deletion Size | 11,406 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 17
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 36,898,708 | 36,898,728 | TCCAAGGACCACAAGTCTCAC |
| TUP | 36,898,733 | 36,898,757 | TCACCTTGATAGATGAGGCTGCCCA |
| TUR | 36,898,758 | 36,898,778 | CAGGTGCCTACCTTGTAGTTC |
| TDF | 36,909,477 | 36,909,494 | ATGGGCACTGCGGATCTC |
| TDP | 36,909,497 | 36,909,522 | CCTCTGGATGCCAGCCATTCACCAAT |
| TDR | 36,909,592 | 36,909,613 | TGGCATCATAGAAGACGACCTC |
PCR Primers
| Primer | Sequence |
| SU | ACTCTCGGTTTCTGTTCTTG |
| SD | AGAGAAAGCAGCAGCCAATG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 71 bp | + | + | - |
| TDF | TDR | 137 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14440B-C4 | VGB6 | C57BL/6NTac | |
| 14440C-B6 | VGB6 | C57BL/6NTac | |
| 14440C-F6 | VGB6 | C57BL/6NTac | |
| 14440C-F11 | VGB6 | C57BL/6NTac | |
| 14440C-A8 | VGB6 | C57BL/6NTac | |
