| Gene | Slc5a5 |
| Chromosome | 8 |
| Deletion Start | 70,892,658 |
| Deletion End | 70,883,782 |
| Deletion Size | 8,877 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 8
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 70,892,392 | 70,892,413 | CGGTCTCAAGTTTCTGTGGATG |
| TUP | 70,892,369 | 70,892,391 | TGCGTGGGCCAGTTGCTCAATTC |
| TUR | 70,892,343 | 70,892,362 | GCAAGAAGAGCAAGGCTGTG |
| TDF | 70,884,582 | 70,884,603 | TGTAGAGCCAGTCGATGATCTG |
| TDP | 70,884,553 | 70,884,577 | TTGGAGACACTGTCACCTCAGGTCA |
| TDR | 70,884,530 | 70,884,551 | TCCTTTGTGTCAATCTGGTCAC |
PCR Primers
| Primer | Sequence |
| SU | GGAACAAGCCCTAGATGTGG |
| SD | GGAAGCTTCACAATCCTTGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 71 bp | + | + | - |
| TDF | TDR | 74 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14441A-F9 | VGB6 | C57BL/6NTac | |
