14446   Tbx22     Status: ES cell colonies screened / QC positives

GeneTbx22
ChromosomeX
Deletion Start107,676,866
Deletion End107,685,460
Deletion Size8,595
Genome Build38
MGI IDMGI:2389465
NCBI Gene ID245572
BAC ID392M1
BAC LibrarybMQ
CassetteZEN-Ub1
Clones AvailableYes

Primer Genomic Coordinates on Chromosome X

Please be aware that these oligonucleotides have been validated only by Taqman assay.

PrimerStartEndSequence
TUF107,677,658107,677,677GAGCAACAGCCAGGAAAGTC
TUP107,677,678107,677,707TGCAAGAAGAAAGTGTTATCCAAGTGGAGC
TUR107,677,730107,677,750GTGCCAATGTCGTGGAATCTC
TDF107,684,841107,684,858TCCCAGGTGGAAGCAGTG
TDP107,684,866107,684,889TCCAGTGACCTCTAGTGGAGGAGC
TDR107,684,912107,684,935AGGTGGAGAACAAGATGGAGAAAG

PCR Primers

PrimerSequence
SUCTGATGGTACACACATGCCC
SDATTTGTAGACTTAACTTCTGGC
LacInRTTGACTGTAGCGGCTGATGTTG
LacInfGGTAAACTGGCTCGGATTAGGG
NeoInRTACTTTCTCGGCAGGAGCAAGGTG
NeoInFTTCGGCTATGACTGGGCACAACAG
LacInZRevGTCTGTCCTAGCTTCCTCACTG
NeoFwdTCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward PrimerReverse PrimerLengthWTHETKO
SULacZRev bp-++
TUFTUR93 bp++-
TDFTDR95 bp++-
NeoFwdSD bp-++
LacInfLacInR210 bp-++
NeoInfNeoInR282 bp-++

Available Clones

ES Cell ordering information can be found at the KOMP Repository.
Clone NameParental ES CellMouse StrainCassette Copy Number
14446B-C4VGB6C57BL/6NTac
14446B-D10VGB6C57BL/6NTac
14446B-E3VGB6C57BL/6NTac
14446B-G3VGB6C57BL/6NTac
14446C-B3VGB6C57BL/6NTac
VELOCIGENE KOMP DESIGN DIAGRAMS
VelociGene KOMP Design
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VelociGene Genotyping Strategy

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