| Gene | Cyp11a1 |
| Chromosome | 9 |
| Deletion Start | 58,015,042 |
| Deletion End | 58,026,942 |
| Deletion Size | 11,901 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 9
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 58,015,642 | 58,015,661 | GACATTGGTGCCCTGACTAC |
| TUP | 58,015,670 | 58,015,691 | CCCGACCAGGCTTTAGCGCCAG |
| TUR | 58,015,711 | 58,015,729 | TTGCTCAGCTCCCAGACAC |
| TDF | 58,026,249 | 58,026,266 | TGCGGAGCTGGAGATGAC |
| TDP | 58,026,270 | 58,026,299 | CCTCCTTATCAATGTGAGTAGGGCTCTGGG |
| TDR | 58,026,335 | 58,026,358 | CTGACAGAGGAGGGTTATGACTTC |
PCR Primers
| Primer | Sequence |
| SU | CAGGAGCTGAGGTTGAGACC |
| SD | TCCTTACAATCCCACAAGGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 88 bp | + | + | - |
| TDF | TDR | 110 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14449A-H6 | VGB6 | C57BL/6NTac | |
| 14449A-E1 | VGB6 | C57BL/6NTac | |
| 14449A-E4 | VGB6 | C57BL/6NTac | |
| 14449A-G5 | VGB6 | C57BL/6NTac | |
| 14449A-E7 | VGB6 | C57BL/6NTac | |
| 14449A-D10 | VGB6 | C57BL/6NTac | |
