| Gene | Ranbp2 |
| Chromosome | 10 |
| Deletion Start | 58,446,983 |
| Deletion End | 58,480,815 |
| Deletion Size | 33,833 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 10
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 58,447,446 | 58,447,469 | CACGGAAGGCCCATAATTTAAGTC |
| TUP | 58,447,471 | 58,447,492 | TCTAGTGCTGTGCCCCGCTTCC |
| TUR | 58,447,526 | 58,447,545 | TGCAACTACCGCCTAGAAGG |
| TDF | 58,480,659 | 58,480,681 | CAGAGTCCAGAGTGGATCTGAAG |
| TDP | 58,480,690 | 58,480,715 | AAACCCGACAAATGTGAGCTGCCACA |
| TDR | 58,480,716 | 58,480,739 | GAAGATTGCTTGATGTCAGAGTTC |
PCR Primers
| Primer | Sequence |
| SU | TTTGTGACGTAGCACCAGTG |
| SD | TCAGTAACACATGCTTCAAGTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 100 bp | + | + | - |
| TDF | TDR | 81 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14451B-F6 | VGB6 | C57BL/6NTac | |
