| Gene | Sp2 |
| Chromosome | 11 |
| Deletion Start | 96,977,624 |
| Deletion End | 96,954,444 |
| Deletion Size | 23,181 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 96,976,874 | 96,976,897 | ATCGCTCGTAACAGATGACCTAAG |
| TUP | 96,976,833 | 96,976,857 | TTTCTTCATCCGCGTTGCTGGCTTA |
| TUR | 96,976,783 | 96,976,806 | AAGGCACTGGGAAAGAAACCTAAG |
| TDF | 96,954,834 | 96,954,855 | TGGGACACAGTCCAGAATGAAG |
| TDP | 96,954,806 | 96,954,832 | AGGTATGCAGAGGCTCAAGGACAAGCA |
| TDR | 96,954,771 | 96,954,789 | TCCGTTCTTGGGTACACTC |
PCR Primers
| Primer | Sequence |
| SU | TGTTGCTGTCCATCAACCTC |
| SD | CTCCTCGACGGAGTGAAATG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 115 bp | + | + | - |
| TDF | TDR | 85 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14463A-D1 | VGB6 | C57BL/6NTac | |
| 14463A-D6 | VGB6 | C57BL/6NTac | |
| 14463A-G9 | VGB6 | C57BL/6NTac | |
| 14463A-G1 | VGB6 | C57BL/6NTac | |
