14465   Ada     Status: ES cell colonies screened / QC no positives

GeneAda
Chromosome2
Deletion Start163,750,088
Deletion End163,727,484
Deletion Size22,605
Genome Build38
MGI IDMGI:87916
NCBI Gene ID11486
BAC ID386M23
BAC LibrarybMQ
CassetteZEN-Ub1
Clones AvailableNo

Primer Genomic Coordinates on Chromosome 2

Please be aware that these oligonucleotides have been validated only by Taqman assay.

PrimerStartEndSequence
TUF163,749,382163,749,402CTGTCTTTCTGCAAGCTGTGG
TUP163,749,353163,749,379TCTTGGAAACAGGGAGACTCCAGGTCC
TUR163,749,325163,749,346TCTGTAAGGGCTGCAAATCTGG
TDF163,727,596163,727,617CCTCCCAGAGGAAGAGAAGAAG
TDP163,727,564163,727,591TTCTGGAACGGCTCTACAGAGAATACCA
TDR163,727,533163,727,551CACAAGCGTACCGTCAGTC

PCR Primers

PrimerSequence
SUCTGCCTTGTGATTCTGCAAC
SDACTCATGGAACTGGGACTGG
LacInRTTGACTGTAGCGGCTGATGTTG
LacInfGGTAAACTGGCTCGGATTAGGG
NeoInRTACTTTCTCGGCAGGAGCAAGGTG
NeoInFTTCGGCTATGACTGGGCACAACAG
LacInZRevGTCTGTCCTAGCTTCCTCACTG
NeoFwdTCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward PrimerReverse PrimerLengthWTHETKO
SULacZRev bp-++
TUFTUR78 bp++-
TDFTDR85 bp++-
NeoFwdSD bp-++
LacInfLacInR210 bp-++
NeoInfNeoInR282 bp-++
VELOCIGENE KOMP DESIGN DIAGRAMS
VelociGene KOMP Design
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VelociGene Genotyping Strategy

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