| Gene | 1810031K17Rik |
| Chromosome | 1 |
| Deletion Start | 75,141,481 |
| Deletion End | 75,136,320 |
| Deletion Size | 5,162 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 1
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 75,141,300 | 75,141,320 | CCCTGGAGTGGGCTATTGTTG |
| TUP | 75,141,277 | 75,141,299 | AGAGCAATGCCACTTGGGCCTGA |
| TUR | 75,141,228 | 75,141,251 | CCCAGTCTAATCACTAGCAGTTCC |
| TDF | 75,136,916 | 75,136,933 | GCTGTCCTGCCTGTTAGC |
| TDP | 75,136,884 | 75,136,909 | ATATGTGAGCAGCGTGGCCCTAGCTG |
| TDR | 75,136,856 | 75,136,880 | GACTGGTGTATTACAGCCACTGATG |
PCR Primers
| Primer | Sequence |
| SU | ACCACGACTACAAAGCCTGG |
| SD | GGTCCTCAGCAGCTCTCATC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 93 bp | + | + | - |
| TDF | TDR | 78 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14467A-C5 | VGB6 | C57BL/6NTac | |
| 14467A-E4 | VGB6 | C57BL/6NTac | |
| 14467A-F7 | VGB6 | C57BL/6NTac | |
| 14467A-H10 | VGB6 | C57BL/6NTac | |
| 14467A-D1 | VGB6 | C57BL/6NTac | |
