| Gene | Trmt12 |
| Chromosome | 15 |
| Deletion Start | 58,872,758 |
| Deletion End | 58,874,113 |
| Deletion Size | 1,356 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 15
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 58,872,773 | 58,872,796 | GAGTCTGTAGTTGTCGCAGTTGTG |
| TUP | 58,872,804 | 58,872,826 | CCCGGTTCACGCAGAGATACCGA |
| TUR | 58,872,827 | 58,872,845 | TCTGCTCCTCCAGGTAGTC |
| TDF | 58,873,473 | 58,873,493 | TGCCCTTCCTAGTTCATGCTG |
| TDP | 58,873,495 | 58,873,520 | CGCTGCCTTCGTACATGCCTGTGAAT |
| TDR | 58,873,559 | 58,873,580 | GGTCTGCCACTCCATTGATCTC |
PCR Primers
| Primer | Sequence |
| SU | AACAATAAGGTGGCTGGTGC |
| SD | CACGATCCAATTCAAGGAGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 73 bp | + | + | - |
| TDF | TDR | 108 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14470A-C5 | VGB6 | C57BL/6NTac | |
| 14470A-E9 | VGB6 | C57BL/6NTac | |
| 14470A-D6 | VGB6 | C57BL/6NTac | |
