| Gene | Cabin1 |
| Chromosome | 10 |
| Deletion Start | 75,755,368 |
| Deletion End | 75,726,863 |
| Deletion Size | 28,506 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 10
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 75,754,789 | 75,754,809 | ATGCACTCTGAGGTCTGCTTC |
| TUP | 75,754,764 | 75,754,788 | CGAGGAAGCTCCTGCTGCTCAGCTT |
| TUR | 75,754,738 | 75,754,759 | CCTCTGAGGACACAAGGGATTC |
| TDF | 75,727,269 | 75,727,287 | CAAATGCTGGCGTGGTGTG |
| TDP | 75,727,236 | 75,727,262 | TCAGGTGGGTCAGTCATATGCTTCAGA |
| TDR | 75,727,205 | 75,727,223 | ACTGCTCTGGCTGGCTCTG |
PCR Primers
| Primer | Sequence |
| SU | CCTGTTTTGTTTTTCCCTTCC |
| SD | TCATGGCATTAGCCATGAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 72 bp | + | + | - |
| TDF | TDR | 83 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14475A-C6 | VGB6 | C57BL/6NTac | |
