14478   Cast     Status: ES cell colonies screened / QC positives

GeneCast
Chromosome13
Deletion Start74,807,845
Deletion End74,780,507
Deletion Size27,339
Genome Build38
MGI IDMGI:1098236
NCBI Gene ID12380
BAC ID
BAC Library
CassetteZEN-Ub1
Clones AvailableYes

Primer Genomic Coordinates on Chromosome 13

Please be aware that these oligonucleotides have been validated only by Taqman assay.

PrimerStartEndSequence
TUF74,807,72774,807,747TCCAGAAGTTGGAGGGTCAAG
TUP74,807,70274,807,724CAGGGACCCGGTGAGACCTCCTT
TUR74,807,67574,807,695CTGCCCGAGCTTAATCAGAAG
TDF74,780,92774,780,949TCTGTTGAACCACAAGAAAGGAG
TDP74,780,90374,780,925CAGCCACTGCAGAGGGAGTGACA
TDR74,780,85874,780,880TCACCTGAGAAGCCATCATTTTG

PCR Primers

PrimerSequence
SUCACCTTCCAGAGTGGGAAAG
SDCACCCTCTCTTCCAACACAA
LacInRTTGACTGTAGCGGCTGATGTTG
LacInfGGTAAACTGGCTCGGATTAGGG
NeoInRTACTTTCTCGGCAGGAGCAAGGTG
NeoInFTTCGGCTATGACTGGGCACAACAG
LacInZRevGTCTGTCCTAGCTTCCTCACTG
NeoFwdTCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward PrimerReverse PrimerLengthWTHETKO
SULacZRev bp-++
TUFTUR73 bp++-
TDFTDR92 bp++-
NeoFwdSD bp-++
LacInfLacInR210 bp-++
NeoInfNeoInR282 bp-++

Available Clones

ES Cell ordering information can be found at the KOMP Repository.
Clone NameParental ES CellMouse StrainCassette Copy Number
14478A-E9VGB6C57BL/6NTac
14478B-H12VGB6C57BL/6NTac
14478C-A7VGB6C57BL/6NTac
14478C-C6VGB6C57BL/6NTac
VELOCIGENE KOMP DESIGN DIAGRAMS
VelociGene KOMP Design
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VelociGene Genotyping Strategy

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