| Gene | Fzd7 |
| Chromosome | 1 |
| Deletion Start | 59,482,963 |
| Deletion End | 59,484,718 |
| Deletion Size | 1,756 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 1
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 59,483,759 | 59,483,778 | TCTTCACGGTGCTCACCTAC |
| TUP | 59,483,780 | 59,483,802 | TAGTGGACATGCGTCGCTTCAGC |
| TUR | 59,483,820 | 59,483,839 | AGCAACCCGACAGGAAGATG |
| TDF | 59,484,368 | 59,484,387 | GCGTCTTCAGCGTGCTCTAC |
| TDP | 59,484,388 | 59,484,407 | ACGGTGCCGGCCACCATCGT |
| TDR | 59,484,440 | 59,484,458 | GTGCGTTCCCAGTGCTCTC |
PCR Primers
| Primer | Sequence |
| SU | GGAGAAGCTTGTTGCTCGTC |
| SD | AGAACAACCACTTGCCTTGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 81 bp | + | + | - |
| TDF | TDR | 91 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14487A-D11 | VGB6 | C57BL/6NTac | |
| 14487A-H7 | VGB6 | C57BL/6NTac | |
| 14487A-C7 | VGB6 | C57BL/6NTac | |
| 14487A-G10 | VGB6 | C57BL/6NTac | |
