| Gene | Pcdh18 |
| Chromosome | 3 |
| Deletion Start | 49,756,861 |
| Deletion End | 49,744,583 |
| Deletion Size | 12,279 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 3
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 49,756,139 | 49,756,161 | GCATTTCGGATTCCAACGACAAC |
| TUP | 49,756,118 | 49,756,138 | AGCCCGGCCTTTGAGCAGCCT |
| TUR | 49,756,065 | 49,756,086 | TCAAGGAGCAAGGTTCCAACTG |
| TDF | 49,744,770 | 49,744,790 | TCACTCTAGTGTGCAGCCTTC |
| TDP | 49,744,745 | 49,744,768 | TCCAAGTGGCTGCCAGCTATGGAG |
| TDR | 49,744,661 | 49,744,682 | GGCATCCATGAGTTCGTGTTTC |
PCR Primers
| Primer | Sequence |
| SU | ACTGGAGTCAGCAATCACCG |
| SD | AAAGGAAGTAGCCTCGTTTAATG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 97 bp | + | + | - |
| TDF | TDR | 130 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14494A-C1 | VGB6 | C57BL/6NTac | |
| 14494A-H11 | VGB6 | C57BL/6NTac | |
