| Gene | Hoxa2 |
| Chromosome | 6 |
| Deletion Start | 52,164,641 |
| Deletion End | 52,162,847 |
| Deletion Size | 1,795 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 6
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 52,163,813 | 52,163,833 | GGCAGGAATTTGATGGAGGTG |
| TUP | 52,163,789 | 52,163,810 | AGAGACTGGGCTTCCCCGGGCT |
| TUR | 52,163,749 | 52,163,769 | GGATGCAGTGCAGCTATTGTG |
| TDF | 52,163,083 | 52,163,103 | GTGGAGCTGGCCTAAACAATG |
| TDP | 52,163,060 | 52,163,082 | ACAGTCCCGAGGCCATCGAGGTC |
| TDR | 52,163,014 | 52,163,033 | GCAGGCAGGAATCTGTGGAG |
PCR Primers
| Primer | Sequence |
| SU | CCAGGGAGCACACTAGATCC |
| SD | CAGTATGAGTCTTGAGAAAGAGGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 85 bp | + | + | - |
| TDF | TDR | 90 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14499A-E10 | VGB6 | C57BL/6NTac | |
| 14499A-G6 | VGB6 | C57BL/6NTac | |
| 14499A-H9 | VGB6 | C57BL/6NTac | |
| 14499A-B10 | VGB6 | C57BL/6NTac | |
