| Gene | Hrg |
| Chromosome | 16 |
| Deletion Start | 22,951,159 |
| Deletion End | 22,961,584 |
| Deletion Size | 10,426 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 16
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 22,951,311 | 22,951,329 | GGTCTCTGACGCCCACTTG |
| TUP | 22,951,332 | 22,951,357 | CAGAGCGGTAAGACATGGCCAATGGA |
| TUR | 22,951,375 | 22,951,395 | CCTTTCCCACCTTCCACATTG |
| TDF | 22,961,219 | 22,961,243 | GGACATGACTTCCTTGACTATGGAC |
| TDP | 22,961,246 | 22,961,268 | TGTGACCCGCCCTCCAATAGTCA |
| TDR | 22,961,270 | 22,961,293 | CCGATGATACTGACCTTTGAGTTC |
PCR Primers
| Primer | Sequence |
| SU | TCCACAGTCACAGCTGAAGG |
| SD | AACATCTTTCCTCTCATCTCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 85 bp | + | + | - |
| TDF | TDR | 75 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14509A-B1 | VGB6 | C57BL/6NTac | |
| 14509A-C7 | VGB6 | C57BL/6NTac | |
| 14509A-C8 | VGB6 | C57BL/6NTac | |
| 14509A-D5 | VGB6 | C57BL/6NTac | |
| 14509A-H4 | VGB6 | C57BL/6NTac | |
| 14509A-E5 | VGB6 | C57BL/6NTac | |
