| Gene | 2410016O06Rik |
| Chromosome | 12 |
| Deletion Start | 83,950,695 |
| Deletion End | 83,952,538 |
| Deletion Size | 1,844 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 12
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 83,951,282 | 83,951,303 | CAGCTACTACGAGGGTCTCTTC |
| TUP | 83,951,304 | 83,951,323 | TCCACCGCCGACCTGGACTC |
| TUR | 83,951,345 | 83,951,363 | CAGATGCTGCCCGAATTGC |
| TDF | 83,951,666 | 83,951,684 | GGACCCGAGTGAGGAGTTG |
| TDP | 83,951,687 | 83,951,713 | CCTGACATCTAGTCCCAACTTCAGCCA |
| TDR | 83,951,739 | 83,951,759 | TCCAGGTTCCAGTACCGTTTG |
PCR Primers
| Primer | Sequence |
| SU | TTGAAATGCCTCGTGCCTTC |
| SD | AACTTAACGCGCAGCACAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 82 bp | + | + | - |
| TDF | TDR | 94 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14511A-D2 | VGB6 | C57BL/6NTac | |
| 14511A-E8 | VGB6 | C57BL/6NTac | |
| 14511A-D7 | VGB6 | C57BL/6NTac | |
| 14511A-E3 | VGB6 | C57BL/6NTac | |
