| Gene | Upk3a |
| Chromosome | 15 |
| Deletion Start | 85,017,223 |
| Deletion End | 85,022,470 |
| Deletion Size | 5,248 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 15
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 85,018,060 | 85,018,080 | CTTTGCCACCAACAACCCTAC |
| TUP | 85,018,081 | 85,018,102 | CCTCACCACCGTGGCCTTGGAG |
| TUR | 85,018,107 | 85,018,129 | TGAGCTATCAAACATGCACAGAG |
| TDF | 85,021,516 | 85,021,537 | GCAGACAGAGCCTTGGTATCTC |
| TDP | 85,021,538 | 85,021,560 | AGGGTCACGTCCTTCAGCTGGCA |
| TDR | 85,021,585 | 85,021,605 | TAACCGGGATGCACTAGAGTC |
PCR Primers
| Primer | Sequence |
| SU | GAGAATTAGCGTCTTTGCGG |
| SD | GATCATTCTCCAGAGGGCAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 70 bp | + | + | - |
| TDF | TDR | 90 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14512A-A6 | VGB6 | C57BL/6NTac | |
| 14512A-C11 | VGB6 | C57BL/6NTac | |
| 14512A-C12 | VGB6 | C57BL/6NTac | |
| 14512A-D3 | VGB6 | C57BL/6NTac | |
| 14512A-E11 | VGB6 | C57BL/6NTac | |
| 14512A-G7 | VGB6 | C57BL/6NTac | |
