| Gene | Bhlhe23 |
| Chromosome | 2 |
| Deletion Start | 180,776,740 |
| Deletion End | 180,775,997 |
| Deletion Size | 744 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 180,776,685 | 180,776,703 | CGCTGAGCCACAGCTACAC |
| TUP | 180,776,659 | 180,776,678 | CGGCCACGCCTACGCTGCAG |
| TUR | 180,776,616 | 180,776,633 | GGACCCGATGCACCGAAG |
| TDF | 180,776,120 | 180,776,139 | CAGACAAGTGTGCCACCTTC |
| TDP | 180,776,096 | 180,776,117 | AGGATCGCCCTCGGCGCTTTGC |
| TDR | 180,776,015 | 180,776,033 | CAGCGAGGACAGGGAACAG |
PCR Primers
| Primer | Sequence |
| SU | GAACAAGGCAAGACTGGAGC |
| SD | AGTCTTGGAGCCAACAGAGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 88 bp | + | + | - |
| TDF | TDR | 125 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14518A-B6 | VGB6 | C57BL/6NTac | |
| 14518A-D3 | VGB6 | C57BL/6NTac | |
| 14518A-D11 | VGB6 | C57BL/6NTac | |
| 14518A-G4 | VGB6 | C57BL/6NTac | |
| 14518A-B2 | VGB6 | C57BL/6NTac | |
