| Gene | Csl |
| Chromosome | 10 |
| Deletion Start | 99,759,198 |
| Deletion End | 99,757,642 |
| Deletion Size | 1,557 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 10
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 99,759,138 | 99,759,156 | CCACCCTGCCTTGTCCTTG |
| TUP | 99,759,102 | 99,759,129 | CATGCCAGTGCTTCTTCCACGAACTTGA |
| TUR | 99,759,063 | 99,759,082 | TGGCTTGCTCCTTGGGTATC |
| TDF | 99,758,380 | 99,758,402 | GTGGTAATGTAAGTGCCCACACC |
| TDP | 99,758,357 | 99,758,379 | AGCCATTTGGTGGGCAGTGCCCT |
| TDR | 99,758,274 | 99,758,296 | CAAGCACCTCCTGATTTGCTAGC |
PCR Primers
| Primer | Sequence |
| SU | GGGAGAAGTTCTAAACCGCC |
| SD | ATCCACTATGATTTCTATGGAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 94 bp | + | + | - |
| TDF | TDR | 129 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14519A-G5 | VGB6 | C57BL/6NTac | |
| 14519A-B9 | VGB6 | C57BL/6NTac | |
| 14519A-B11 | VGB6 | C57BL/6NTac | |
| 14519A-G2 | VGB6 | C57BL/6NTac | |
