| Gene | Adck3 |
| Chromosome | 1 |
| Deletion Start | 180,182,235 |
| Deletion End | 180,167,114 |
| Deletion Size | 15,122 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 1
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 180,181,762 | 180,181,780 | GGCCCATTTCCTGGCTCTG |
| TUP | 180,181,724 | 180,181,752 | TGTAATCTCCTTCCATGTACTGGCATGCA |
| TUR | 180,181,697 | 180,181,719 | GGACTGCATGTCTGAAACCTAAC |
| TDF | 180,167,257 | 180,167,278 | TGGGTGGCTCTTTCCTCATCTG |
| TDP | 180,167,233 | 180,167,255 | TCTAAGCTGAAGGCCCGCTTCCC |
| TDR | 180,167,209 | 180,167,230 | GCTTCCTCGAACATGGCCTTAC |
PCR Primers
| Primer | Sequence |
| SU | TGCACATGCTAAGTCTTGCC |
| SD | AAAGGGTCTGTTGTCCATGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 84 bp | + | + | - |
| TDF | TDR | 70 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14522A-G7 | VGB6 | C57BL/6NTac | |
| 14522A-D9 | VGB6 | C57BL/6NTac | |
| 14522A-F3 | VGB6 | C57BL/6NTac | |
| 14522A-E9 | VGB6 | C57BL/6NTac | |
| 14522A-A10 | VGB6 | C57BL/6NTac | |
