| Gene | Mixl1 |
| Chromosome | 1 |
| Deletion Start | 180,697,009 |
| Deletion End | 180,694,573 |
| Deletion Size | 2,437 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 1
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 180,696,425 | 180,696,444 | GCTTGCGGACCCATTGAAAG |
| TUP | 180,696,399 | 180,696,423 | TCCCAGCAGGAAGGTAGTCATTGCA |
| TUR | 180,696,379 | 180,696,398 | TACTGGACGCTGGGAAAGAG |
| TDF | 180,694,819 | 180,694,836 | TCCATTGCCCTGCTCCTG |
| TDP | 180,694,789 | 180,694,816 | ACTGAAGCTAGGTGTTTGAAGCCTCAGC |
| TDR | 180,694,756 | 180,694,776 | CCGGAACGTGGTTCACATCTG |
PCR Primers
| Primer | Sequence |
| SU | CTTGAGAGGTCTTCTGGCCC |
| SD | TGGGATAGAGTGTGTGAACCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 66 bp | + | + | - |
| TDF | TDR | 81 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14523A-A9 | VGB6 | C57BL/6NTac | |
| 14523A-B2 | VGB6 | C57BL/6NTac | |
| 14523A-H9 | VGB6 | C57BL/6NTac | |
| 14523A-H5 | VGB6 | C57BL/6NTac | |
| 14523A-G5 | VGB6 | C57BL/6NTac | |
